Anticorps > 20421 - Purified freeze-dried antibody to bovine type IV collagen
20421 - Purified freeze-dried antibody to bovine type IV collagen
Bovine skin paraffin section, IHC
species
Bovine
immunogen
Type IV collagen
Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain. Mutations in COL4A5 are associated with Alport syndrome. [according to RefSeq]
Immunogen : Type IV collagen extracted from bovine lens capsules.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #326f
-
IgG
1,7 mg/mL
pH
7,5
ELISA
Optimal working dilution not tested with our current protocol.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen bovine skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit,0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded bovine skin.
-
Presentation
Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain. Mutations in COL4A5 are associated with Alport syndrome. [according to RefSeq]
Description
Immunogen : Type IV collagen extracted from bovine lens capsules.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #326f
-
IgG :
1,7
pH :
7,5
ELISA
Optimal working dilution not tested with our current protocol.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen bovine skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit,0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded bovine skin.
-
References
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Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A. - «Myofibroblastic stromal reaction in carcinoma of the breast : variations of collagenous matrix and structural glycoproteins. Virchows Arch. 1985, 408, 49-59.»
Lagacé R., Grimaud J.A., Schürch W., Seemayer T.A. - «A procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver. J. Histochem. Cytochem. 1985, 33, 407-414.»
Clement B., Rissel M., Peyrol S., Mazurier Y., Grimaud J.A., Guillouzo A. - «Mise en évidence du collagène par immunofluorescence au cours du développement du Xénope, Xenopus laevis Daud. CR Soc. Biol. 1982, 176, 494-502.»
Bride M., Benslimane S., Stocker S., Grimaud J.A.
