20251 - Purified freeze-dried antibody to mouse type II collagen

Type II collagen is a fibrillar collagen composed of three identical α₁ (II) chains.  The type II collagen is found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. [according to RefSeq]

Immunogen : Native type II collagen extracted from mouse.

Host : Rabbit.

Polyclonal antibodies purified by chromatography.

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

Reconstituted 

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, IB/WB, SP(RIA).

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user. 

Cross-reactivity with bovine type II collagen, murine type II collagen and murine procollagen.

• #320k

  • IgG 9,3 mg/mL pH 7,5

    ELISA Optimal working dilution not tested with our current protocol. IF With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/80 on frozen mouse cartilage tissues. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse cartilage.

• #320l

  • IgG 8,1 mg/mL pH 7,5

    ELISA Optimal working dilution not tested with our current protocol. IF Optimal working dilution not tested. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse cartilage.

• #373i

  • IgG 3.3 mg/mL pH 7.3

    ELISA Optimal working dilution at 1/2000. IF IF not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse cartilage.